The effects of meal feeding of calcium,protein and energy on production and calcium status of laying hens

1. Laying birds were allowed free access to a basal diet (treatment 1) or were restricted to either early morning and late afternoon access to the basal diet (treatment 2) or a high‐energy diet in the morning and a high‐protein, high‐calcium diet in the afternoon (treatment 3). The sum of the components fed in treatment 3 was equivalent to the basal diet. These diets were fed for 5 weeks. In the next phase of the experiment, which lasted for 3 weeks, the compositions of the diets for treatments 2 and 3 were altered so that the intakes of dietary components approached those of birds on treatment 1.

2. In both experimental phases birds on treatment 3 produced significantly fewer eggs of smaller mean weight and shell weight. Plasma calcium, inorganic phosphorus concentrations and alkaline phosphatase activity were indicative of decreased calcium status.     

The spoilage flora and shelf‐life of duck carcasses stored at 2 or ‐ 1°c in oxygen‐permeable or oxygen‐impermeable film

1. A comparison was made of” oven ready “ duck carcasses stored at 2 or — 1 °C and wrapped in either a low‐density oxygen‐permeable polyethylene film or a heat‐shrunk oxygen‐impermeable film.

2. At spoilage the main organisms at 2 and — 1 °C on the carcasses wrapped in the oxygen‐permeable film were pseudomonads, producing unacceptable “off odours” when their numbers were >10⁸/cm². This occurred in about 10 d at 2 °C but in about 19 d at ‐ 1° C.

3. The effect of wrapping in the heat‐shrunk oxygen‐impermeable film was to delay or inhibit the growth of pseudomonads and thus extend the shelf‐life by more than 50% at either temperature. The predominant organisms isolated from the spoiling carcasses were atypical lactobacilli and enterobacteria.

4. Sensory assessment of the carcasses stored at — 1 °C by a trained panel indicated that, although less obvious “ off odours “ were produced by the micro‐organisms growing on the carcasses wrapped in the impermeable film, differences were detected at 33 d when the numbers of bacteria reached about 10⁷/cm² whilst at 41 d the meat was described as rancid.     

Mucopolysaccharidosis in a domestic short-haired cat--a disease distinct from that seen in the Siamese cat.

A 10-month-old male domestic short-haired cat was examined because of progressive lameness, a broad face with depressed nasal bridge, small ears, corneal clouding, and multiple bone dysplasia. The cat excreted excessive amounts of glycosaminoglycan (a component of connective tissue) in its urine and had evidence of lysosomal storage of glycosaminoglycans in fibroblasts and neurons. Activity of alpha-L-iduronidase, a lysosomal enzyme involved in glycosaminoglycan degradation, was deficient in cultured fibroblasts and leukocytes. The mucopolysaccharidosis was distinct from that seen in Siamese cats in terms of the pathologic changes and the specific enzyme deficiency.     

Isolation of picornavirus from feces and semen from an infertile bull.

Virus was isolated from semen and fecal samples from a bull with orchitis, testicular degeneration, aspermatogenesis, and loss of libido. Both isolates were classified as picornavirus, bovine enterovirus serotype I, on the basis of physical, chemical, and serologic characteristics. Veterinary practitioners that may suspect viral infection as a cause of bovine infertility should submit both semen and fecal samples for virus isolation and identification.     

A seroepizootiologic study of five viruses in a swine-evaluation station.

This serologic study was done to gain information on the spread, maintenance, and effect upon performance of five porcine viruses. Blood samples were taken from two groups of 8- to 11-week-old pigs from a large number of Indiana swine herds in a performance-testing station 1 week after entry, 7 weeks after entry (one group only), and at slaughter. The sera were tested by indirect fluorescent antibody tests for antibodies to transmissible gastroenteritis virus (TGEV), swine influenza virus (SIV), hemagglutinating encephalomyelitis virus (HEV), porcine adenovirus (PAV), and pseudorabies virus (PRV). Seroconversions to TGEV, HEV, and PAV occurred in a group of pigs entered in May and slaughtered in August (group 1). In the group that was entered in October and slaughtered in January (group 2), pigs developed antibodies to SIV, HEV, and PAV, but not to TGEV. Only 1 of the 434 pigs tested had antibodies to PRV, and there were no seroconversions to this virus. The only statistically valid effect of infection on performance was found in group 1 pigs, which had seroconverted to TGEV during the first 7 weeks of their stay. These pigs gained 0.077 kg less per day than pigs that did not develop antibodies to TGEV during that period. The pattern of serologic reactions was indicative of a relatively slow spread of these viruses in the groups. We interpret this as supporting the concept that a relatively slow spread of these viruses through large groups of pigs kept under conditions that are less than optimum for virus spread may be an important means of their interepizootic survival.     

Lymphocyte-associated immune responses to canine distemper and measles viruses in distemper-infected gnotobiotic dogs.

Cellular immune responses of peripheral blood lymphocytes to canine distemper virus and measles virus were determined in vaccinated or infected gnotobiotic dogs, using the technique of syncytia inhibition. Cross-reactivity between viruses was detected in both groups of dogs. Peak responses in vaccinated dogs occurred 11 days after vaccination and declined to base-line levels by 3 weeks, whereas responses in infected dogs were present 30 days after inoculation. Fractionation experiments with peripheral blood lymphocytes indicated that synctia inhibition is probably mediated by T lymphocytes.     

Responses of immune and nonimmune adult rhesus macaques (Macaca mulatta) to adenovirus SV-20.

Adenovirus SV-20 (ASV-20) was inoculated subcutaneously into adult rhesus macaques (Macaca mulatta), and various immunologic parameters were studied. Similar changes were observed in macaques that had anti-ASV-20 serum-neutralizing antibodies prior to inoculation and in those lacking detectable antibodies. There were absolute decreases in numbers of peripheral blood lymphocytes (PBL), erythrocyte-rosetting lymphocytes, complement-receptor lymphocytes, and Fc-receptor lymphocytes. These changes were most significant (P less than 0.05) on postinoculation days (PID) 3 and 7. Mitogenic responsiveness to concanavalin A, phytohemagglutinin, and pokeweed mitogen in cultured PBL from immune and nonimmune macaques was depressed on PID 3, 7, and 14. Ultraviolet-inactivated ASV-20 caused moderate suppression of phytohemagglutinin-induced mitogenesis when viral particles and lectin were added simultaneously to PBL cultures. Plasma cortisol (hydrocortisone) values were not significantly altered following inoculation of ASV-20. High titers of anti-ASV-20 antibody developed by PID 7 in nonimmune macaques, and previously immune macaques showed a booster effect in the same time period. Antibody titers were still increased 120 days after inoculation. There was no clinical evidence of an adverse effect of ASV-20 infection in these macaques.